FIG 6.

Effects of Shp2 depletion on BDNF-induced Erk activation and IEG expression. (A) Immunoblot analysis of Erk1/2 phosphorylation in mouse cortical neurons infected with a lentivirus encoding Shp2 or control shRNA and stimulated with BDNF (50 ng/ml) for 0, 5, 10, or 30 min. Quantitative data are expressed relative to the value for shCtrl-treated cells at time zero. (B) Quantitative PCR analysis of IEG expression in cultured neurons infected as described for panel A and incubated in the absence or presence of BDNF for 60 min. Data are expressed relative to the value for shCtrl-treated cells stimulated with BDNF. Quantitative data are the mean value ± SEM of four independent experiments (A) or five or six culture dishes in three independent experiments (B). *, P < 0.05 (Student's t test) for comparisons between shCtrl- and shShp2-treated neurons; NS, not significant.