FIG 5.
Whi5, Msa1, and Msa2 cooperate to delay CLN2 transcription upon osmostress. Wild-type (wt), triple null (middle), and point mutant (bottom) strains were synchronized in G1 with α-factor and released into YPD medium (control) or 0.4 M NaCl. RNA was extracted from samples taken at the times indicated after release and analyzed by Northern blotting with a radiolabeled probes specific for CLN2, CLB5, or ENO1 mRNA. Quantification relative to the loading control of each mRNA is depicted below each lane. A value of 100% is assigned to the maximum level of a given transcript under control conditions for each strain.