FIG 6.

Whi5, Msa1, and Msa2 are involved in the coordination of budding and DNA replication upon osmostress through Hog1 phosphorylation. (A) The delay in DNA replication initiation upon osmostress does not depend on Whi5, Msa1, or Msa2. The wild-type (wt), triple null, or point mutant cells indicated were synchronized in G₁ with α-factor and released into YPD medium (control) or 0.4 M NaCl. Samples were taken every 10 min after release, and DNA replication was analyzed by flow cytometry. (B) The delay in budding caused by stress depends on Whi5, Msa1, or Msa2. The same cells as in panel A were synchronized with α-factor and released into either YPD medium (control) or 0.4 M NaCl. At the times indicated after release, the cells were mounted on glass slides and budded cells were counted. Bars represent the averages ± the standard deviations from three independent experiments. In each experiment, at least 100 cells were counted at each time point.