Fig. 1.

Cytochalasin B against multiple murine cancer cell lines in vitro. a Effects of 3-day continuous exposure to cytochalasin B on growth of attached and suspension culture murine neoplastic cell lines. Continuous cell lines as indicated in the figure were seeded at 4 to 8 × 10⁴ cells per well (2 to 4 × 10⁴ cells/cm²) for attached cells in 2 ml (triplicate, 24 well-plates, complete medium; see materials and methods), and were allowed to attach overnight. P388/ADR cells were seeded at 5 × 10⁴ cells/ml in 2 ml of medium, and allowed to grow overnight. Cells were continuously exposed to cytochalasin B (CB) at concentrations shown for 3 days at 37 °C in 5 % CO₂ in a humidified atmosphere. Attached cells (B16BL6, B16F10, and M109c) were washed, trypsinized, and counted with a hemacytometer. P388/ADR suspensions were counted with a Coulter counter. The increase in cell number beyond the number present at the time of treatment initiation was compared to the increase in cell number in the corresponding controls. b Effects of short-term, 3 h exposure to cytochalasin B on growth of attached and suspension culture neoplastic cell lines. Cell lines were seeded as detailed in Fig. 1a, but treated with CB at the concentrations shown for only 3 h