Figure 7.

Basophil activation inhibits transendothelial migration. (A) WT C57BL/6, Alox5^−/−, or Ltb4r1^−/− mice were sensitized with anti-DNP IgE and challenged with DNFB by topical application 1 d later. Basophil accumulation was analyzed 3 d after challenge. Each of these lines was bred independently, and the data were pooled from two separate experiments with at least six mice per group. Horizontal bars denote mean. (B and C) Basoph8 mice received IL-3 complex through an intravenous injection, and 3 d later, the spleens were harvested from these animals and placed in a transwell migration assay in the presence (B) or absence (C) of endothelial cells. Data are representative of four independent experiments with duplicate transwells performed each time. (D and E) Basoph8 mice were treated as in B, and the transendothelial cell migration assay was performed in the presence of the dual Pyk2/FAK inhibitor PF 431396 (D) or the selective FAK inhibitor PF 573228 (E). The concentration of each inhibitor is also noted. Data are pooled from two independent experiments each with duplicate transwells at each time point. (A–E) Student’s t tests were performed for statistical analysis: *, P < 0.05; **, P < 0.01.