Figure 8.

shRNA knockdown of Arid3a inhibits FL pro-B generation of B1a B cells. (A) FL pro-B cells or the N38 Abelson mouse line was transduced with empty vector (LMP) or vector containing each of five different shRNAs. 3 d after transduction, GFP⁺ cells were sorted for RNA. ARID expression was quantified by Q-PCR. Arid3a expression was first normalized to β-actin, and then the level in control vector (LMP) was set to 100. Error bars represent ±SEM. (B) Phenotype of CD19⁺GFP⁺ B cells generated in SCID mice 3 wk after transfer of fetal pro-B cells, transduced with empty vector or a pool of three shRNA constructs. The red regions indicate an adult-type and the green boxes indicate a fetal-type phenotype. Data are representative of three independent experiments using two mice per group.