Expression of IL-37b did not affect 4T1 breast carcinoma cell growth in vitro. (A) A total of 2×105 4T1 cells were transduced with Ad-IL37b in each well of 12-well plates. At 48 and 72 h after virus transduction, the amounts of IL-37b in the culture supernatants were detected by ELISA. (B) Cells (1×104) from each subline were seeded in 48-well plates, and cell numbers were counted in triplicate at 24, 36, 48, 60, and 72 h. Mean±SEM. n=3.