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. 2015 Mar 23;36(4):429–439. doi: 10.1038/aps.2015.5

Figure 5.

Figure 5

Chronic nicotine treatment enhances aortic smooth muscle relaxation induced by sodium nitroprusside through cGMP-dependent PKG pathway. Aortic medial relaxation induced by cumulative doses of sodium nitroprusside (SNP) in the absence and presence of soluble guanylyl cyclase inhibitor, ODQ (3 μmol/L) (A) and cGMP dependent protein kinase (PKG) inhibitor, Rp-8-Br-PET-cGMP (30 μmol/L) (C). (B) Aortic medial responses to cumulative doses of cGMP analog, 8-Br-cGMP. (D) Western blot analysis to detect PKG protein expression in aortic media, β-actin expression as a loading control. (E) PKG activity analysis in aortic media. Sal, saline group; Nic, nicotine group; F−E−, both adventitial fat and endothelium removed. Data are shown as mean±SEM. n=3–8. cP<0.01.