Table 4.
Additional assignments for differentially expressed proteins. Fold changes were generated for four comparisons: A1: ET-1 treated vs. basal in Control PASMC; A2. ET-1 treated vs. basal in PAH-BRMP2 PASMC; A3. PAH-BRMP2 cells vs. Control cells under basal condition; A4. PAH-BRMP2 vs. Control cells, both with ET-1 stimulation.
| A. Differentially expressed proteins involved in protein ubiquination. | |||||
|---|---|---|---|---|---|
| Symbol | Entrez Gene Name | Fold Change (A1) |
Fold Change (A2) |
Fold Change (A3) |
Fold Change (A4) |
| DNAJB4 | DnaJ (Hsp40) homolog, subfamily B, member 4 |
1.70 | 2.65 | 4.02 | 6.29 |
| HLA-B | major histocompatibility complex, class I, B |
1.64 | 2.53 | 1.69 | 2.60 |
| HSP90AA1 | heat shock protein 90 kDa alpha (cytosolic), class A member 1 |
1.91 | 2.48 | 1.33 | 1.73 |
| HSP90AB1 | heat shock protein 90 kDa alpha (cytosolic), class B member 1 |
1.91 | 2.40 | 1.31 | 1.65 |
| HSP90B1 | heat shock protein 90 kDa beta (Grp94), member 1 |
1.78 | 2.48 | 1.14 | 1.59 |
| HSPA2 | heat shock 70 kDa protein 2 | 1.83 | 2.49 | 1.12 | 1.52 |
| HSPA5 | heat shock 70 kDa protein 5 (glucose-regulated protein, 78 kDa) | 1.80 | 2.37 | −1.02 | 1.29 |
| HSPA6 | heat shock 70 kDa protein 6 (HSP70B') |
1.85 | 2.16 | 1.53 | 1.79 |
| HSPA8 | heat shock 70 kDa protein 8 | 1.65 | 2.34 | −1.06 | 1.33 |
| HSPA9 | heat shock 70 kDa protein 9 (mortalin) |
1.35 | 1.59 | 1.44 | 1.70 |
| HSPA1L | heat shock 70 kDa protein 1-like | 1.66 | 1.88 | 1.62 | 1.83 |
| HSPD1 | heat shock 60 kDa protein 1 (chaperonin) |
1.89 | 2.36 | 1.31 | 1.63 |
| HSPE1 | heat shock 10 kDa protein 1 (chaperonin 10) |
1.73 | 2.51 | 1.24 | 1.80 |
| PSMA1 | proteasome (prosome, macropain) subunit, alpha type, 1 |
2.10 | 2.89 | 1.30 | 1.79 |
| PSMA2 | proteasome (prosome, macropain) subunit, alpha type, 2 |
1.72 | 1.97 | 1.32 | 1.51 |
| PSMA3 | proteasome (prosome, macropain) subunit, alpha type, 3 |
1.52 | 2.28 | 1.01 | 1.52 |
| PSMA5 | proteasome (prosome, macropain) subunit, alpha type, 5 |
1.99 | 2.45 | 1.36 | 1.68 |
| PSMA6 | proteasome (prosome, macropain) subunit, alpha type, 6 |
1.39 | 2.56 | −1.13 | 1.63 |
| PSMA7 | proteasome (prosome, macropain) subunit, alpha type, 7 |
1.80 | 2.14 | 1.21 | 1.44 |
| PSMB1 | proteasome (prosome, macropain) subunit, beta type, 1 |
1.60 | 1.92 | 1.21 | 1.45 |
| PSMB2 | proteasome (prosome, macropain) subunit, beta type, 2 |
1.42 | 2.57 | −1.01 | 1.80 |
| PSMB3 | proteasome (prosome, macropain) subunit, beta type, 3 |
2.02 | 2.38 | 1.40 | 1.65 |
| PSMC2 | proteasome (prosome, macropain) 26S subunit, ATPase, 2 |
1.50 | 1.97 | 1.07 | 1.40 |
| PSMD8 | proteasome (prosome, macropain) 26S subunit, non-ATPase, 8 |
1.91 | 1.68 | 1.88 | 1.65 |
| PSME1 | proteasome (prosome, macropain) activator subunit 1 (PA28 alpha) |
2.56 | 3.69 | 1.56 | 2.24 |
| PSME2 | proteasome (prosome, macropain) activator subunit 2 (PA28 beta) |
1.72 | 2.17 | 1.30 | 1.64 |
| UBA1 | ubiquitin-like modifier activating enzyme 1 |
1.67 | 2.05 | 1.01 | 1.24 |
| UBE2N | ubiquitin-conjugating enzyme E2N | 1.78 | 2.29 | 1.28 | 1.65 |
| USO1 | USO1 vesicle docking protein homolog (yeast) |
1.97 | 3.58 | −1.10 | 1.65 |
| USP5 | ubiquitin specific peptidase 5 (isopeptidase T) |
1.52 | 1.72 | 1.58 | 1.78 |
| B. Differentially expressed proteins involved in the Glycolysis/Gluconeogenesis pathway. | |||||
|---|---|---|---|---|---|
| Symbol | Entrez Gene Name | Fold Change (A1) |
Fold Change (A2) |
Fold Change (A3) |
Fold Change (A4) |
| ADH5 | alcohol dehydrogenase 5 (class III), chi polypeptide | 1.63 | 2.14 | 1.24 | 1.64 |
| ALDH9A1 | aldehyde dehydrogenase 9 family, member A1 |
1.55 | 2.27 | 2.62 | |
| ALDOA | aldolase A, fructose-bisphosphate | 1.45 | 1.99 | −1.16 | 1.19 |
| ENO1 | enolase 1, (alpha) | 1.46 | 1.87 | 1.03 | 1.32 |
| ENO2 | enolase 2 (gamma, neuronal) | 1.59 | 1.72 | 1.28 | 1.38 |
| ENO3 | enolase 3 (beta, muscle) | 1.60 | 1.71 | 1.27 | 1.37 |
| GPI | glucose-6-phosphate isomerase | 1.57 | 1.98 | 1.31 | 1.66 |
| HK1 | hexokinase 1 | 1.43 | 1.62 | 1.69 | 1.92 |
| LDHA | lactate dehydrogenase A | 1.70 | 2.88 | 1.33 | 2.26 |
| LDHAL6A | lactate dehydrogenase A-like 6A | 1.41 | 2.70 | 1.11 | 2.13 |
| LDHAL6B | lactate dehydrogenase A-like 6B | 2.33 | 3.30 | 1.86 | 2.64 |
| LDHB | lactate dehydrogenase B | 1.49 | 2.59 | 1.18 | 2.05 |
| PFKL | phosphofructokinase, liver | 1.74 | 2.37 | 1.17 | 1.60 |
| PGAM1 | phosphoglycerate mutase 1 (brain) |
1.33 | 2.12 | −1.28 | 1.24 |
| PGK1 | phosphoglycerate kinase 1 | 1.64 | 2.40 | −1.21 | 1.21 |
| PGK2 | phosphoglycerate kinase 2 | 1.75 | 2.42 | −1.15 | 1.20 |
| PGM1 | phosphoglucomutase 1 | 2.09 | 1.92 | 1.97 | 1.81 |
| PKM2 | pyruvate kinase, muscle | 1.69 | 1.74 | 1.10 | 1.13 |
| TPI1 | triosephosphate isomerase 1 | 1.43 | 2.36 | −1.23 | 1.34 |
| C. Differentially expressed proteins involved in the Pentose Phosphate Pathway. | |||||
|---|---|---|---|---|---|
| Symbol | Entrez Gene Name | Fold Change (A1) |
Fold Change (A2) |
Fold Change (A3) |
Fold Change (A4) |
| ALDOA | aldolase A, fructose-bisphosphate | 1.45 | 1.99 | −1.16 | 1.19 |
| G6PD | glucose-6-phosphate dehydrogenase |
1.59 | 2.56 | 1.00 | 1.61 |
| GPI | glucose-6-phosphate isomerase | 1.57 | 1.98 | 1.31 | 1.66 |
| PFKL | phosphofructokinase, liver | 1.74 | 2.37 | 1.17 | 1.60 |
| PGLS | 6-phosphogluconolactonase | 1.38 | 1.90 | 1.41 | 1.94 |
| PGM1 | phosphoglucomutase 1 | 2.09 | 1.92 | 1.97 | 1.81 |
| PRPS1 | phosphoribosyl pyrophosphate synthetase 1 |
1.80 | 2.44 | 1.17 | 1.58 |
| PRPS1L1 | phosphoribosyl pyrophosphate synthetase 1-like 1 |
1.63 | 2.13 | 1.17 | 1.53 |
| TALDO1 | transaldolase 1 | 1.55 | 2.21 | 1.39 | 1.98 |
| TKT | transketolase | 1.52 | 1.67 | 1.33 | 1.46 |
| D. Differentially expressed heat shock proteins. | |||||
|---|---|---|---|---|---|
| Symbol | Entrez Gene Name | Fold Change (A1) |
Fold Change (A2) |
Fold Change (A3) |
Fold Change (A4) |
| HSP90AA1 | heat shock protein 90 kDa alpha (cytosolic), class A member 1 |
1.91 | 2.48 | 1.33 | 1.73 |
| HSP90AB1 | heat shock protein 90 kDa alpha (cytosolic), class B member 1 |
1.91 | 2.39 | 1.31 | 1.65 |
| HSP90B1 | heat shock protein 90 kDa beta (Grp94), member 1 |
1.78 | 2.48 | 1.14 | 1.59 |
| HSPA1L | heat shock 70 kDa protein 1-like | 1.66 | 1.88 | 1.62 | 1.83 |
| HSPA4 | heat shock 70 kDa protein 4 | 1.28 | 1.84 | 1.24 | 1.77 |
| HSPA6 | heat shock 70 kDa protein 6 (HSP70B') |
1.85 | 2.16 | 1.53 | 1.79 |
| HSPA8 | heat shock 70 kDa protein 8 | 1.65 | 2.33 | −1.06 | 1.33 |
| HSPA9 | heat shock 70 kDa protein 9 (mortalin) |
1.35 | 1.59 | 1.44 | 1.70 |
| HSPB1 | heat shock 27 kDa protein 1 | 1.15 | 1.69 | 1.34 | 1.97 |
| HSPB6 | heat shock protein, alpha-crystallin-related, B6 | 1.33 | 1.73 | 2.25 | 2.93 |
| HSPD1 | heat shock 60 kDa protein 1 (chaperonin) |
1.89 | 2.36 | 1.31 | 1.63 |
| HSPE1 | heat shock 10 kDa protein 1 (chaperonin 10) |
1.73 | 2.51 | 1.24 | 1.80 |
| HYOU1 | hypoxia up-regulated 1 | 2.02 | 2.34 | 1.42 | 1.65 |
| SERPINH1 | serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1) |
1.64 | 1.80 | −1.49 | −1.36 |
| E. Expression change of the 14-3-3 family proteins. | |||||
|---|---|---|---|---|---|
| Symbol | Entrez Gene Name | Fold Change (A1) |
Fold Change (A2) |
Fold Change (A3) |
Fold Change (A4) |
| YWHAZ | tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide |
1.85 | 2.76 | 1.23 | 1.84 |
| YWHAQ | tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide |
1.63 | 2.14 | −1.07 | 1.22 |
| YWHAG | tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide |
1.68 | 2.40 | −1.19 | 1.20 |
| YWHAE | tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide |
1.88 | 3.85 | −1.04 | 1.97 |
| YWHAB | tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide |
1.80 | 2.68 | 1.13 | 1.68 |