Skip to main content
. 2015 Mar 13;25(4):401–411. doi: 10.1038/cr.2015.32

Figure 2.

Figure 2

Crystal structure of the Scp1 WD40 domain. (A) Protein purification of the Scp1-WD40 protein (residues 567-961 and 986-1 054, C618S/C671S/C680S/C756S/C873S/C901S/C920S/C941S/C1010S) used for crystallization studies. Two fragments (residues 567-961 and 986-1 054) were co-expressed in E.coli. A representative chromatogram of size exclusion chromatography of the recombinant Scp1-WD40 is shown. Peak fractions were analyzed by SDS-PAGE and visualized by Coomassie blue staining. (B) Overall structure of the Scp1 WD40 domain. Two perpendicular views are shown. The structure is shown in rainbow color, with the N- and C-termini colored blue and red, respectively. The eight blades are numbered 1 to 8 and the four strands in each blade are labeled A to D from the center to the outer ring. All structure figures were prepared with PyMol31.