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. 2015 Apr 2;14:45. doi: 10.1186/s12934-015-0228-2

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© Durairaj et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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5 Significance of homologous FoCYP-FoCPR reconstituted system in the hydroxylation of fatty acids. (A) Reaction profile of hydroxylation of fatty acids by FoCYP539A7 and FoCYP655C2 with the homologous (FoCPR) reductase. (B) Comparative analysis on the catalytic conversion of fatty acids by FoCYP539A7 and FoCYP655C2 with the heterologous (ScCPR) and homologous (FoCPR) reductases. Data were plotted from the 50 hr biotransformation reaction samples. ΔPox1 mutant S. cerevisiae cells harboring the CYP539A7-FoCPR, CYP655C2-FoCPR, CYP39A7-ScCPR and CYP655C2-ScCPR reconstituted systems were induced with 4% galactose, 2 mM 5-ALA and 500 μM of substrates: caprylic acid (C8), capric acid (C10) and lauric acid (C12) were added and cultured at pH 7.0. Samples collected at 10 hr intervals were extracted, trimethylsilyl derivatized and analyzed by GC.