Figure 2.

The Var2-6 FLC allele confers higher FLC expression and later flowering. (A) The Var2-6 FLC haplotype shows a distinct geographical distribution. Forty-six accessions carrying the Var2-6 haplotype are shown as red dots, and 101 accessions carrying other FLC haplotypes are shown as blue dots. The map was generated in R based on the latitude and longitude information of accessions (Supplemental Table S1). (B) Accessions carrying the Var2-6 FLC haplotype are later flowering than accessions carrying other FLC haplotypes. The flowering time of 120 d indicates that the plants did not flower when the experiment was finished. The Kolmogorov-Smirnov (KS) test indicated that the flowering time difference between Var2-6 haplotypes and the other accessions is significant (P < 0.001). (C) FLC expression of the Col allele in plants with and without active FRI. (D) FLC expression of the Var2-6 allele in plants with and without active FRI. Values in C and D are means ± SD from three biological repeats. The fold change in FLC expression between Col and Var2-6 FLC in fri is 28, and the fold change in FRI is 6. (E,F) SNP259T is associated with late flowering in an F2 population generated from a cross between Ka-0 and Ws-0 in fri (E) and FRI (F) homozygotes. The KS test indicated that the flowering time difference between plants containing the Ka-0 FLC allele and the Ws-0 allele is significant (P < 0.05).