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. 2015 Apr 1;29(7):696–701. doi: 10.1101/gad.258814.115

Figure 4.

Figure 4.

G259T heterogeneity affects FLC expression in a COOLAIR-dependent mechanism. (A) The G259T mutagenesis in Col FLC increased FLC expression, as assayed using a pool of 48 independent transgenic lines (Student's t-test, [***] P < 0.001) and 32 randomly selected independent transgenic lines. (B) T259G mutagenesis in Var2-6 FLC decreased FLC expression, as assayed using a pool of 48 independent transgenic lines (Student's t-test, [*] P < 0.05) and 29 randomly selected independent transgenic lines. (C) G259T did not change FLC expression in a COOLAIR terminator exchange construct, as assayed using pools from 21 and 32 independent transgenic lines: Col-TEX and Col-TEX-G259T (Student's t-test, [NS] P = 0.216). (D) Flowering time comparison of Var/FRI flc-2 and Var-T259G/FRI flc-2 of plants given 4 wk of vernalization. Forty-five and 60 independent transgenic lines were scored, with eight individuals of each line included. The KS test indicated that the difference between transgenic lines is significant (P < 0.001). In A–C, values are means ± SD from three biological repeats. All of the transgenic plants shown in A–D were in a FRI flc-2 background.