Figure 6.

lola is mutually trans-spliced with mod(mdg4). (A) Schematic of the lola gene locus in D. melanogaster. All 3′ exons are designated according to isoforms in FlyBase. (Light purple boxes) 5′ alternative exons; (dark purple boxes) common exons; (blue boxes) 3′ alternative exons; (white boxes) internal cis-introns. (B) The conserved TSA core-like motif in the last 5′ intron of lola, which forms 12 bp with U1 snRNA. For sequence alignment of the full-length intron 8 from available Drosophila species, see Supplemental Figure S6. (C) Deletion of a region containing the TSA core-like motif in lola significantly decreases trans-splicing activity. (D) The lola and mod(mdg4) genes are mutually trans-spliced. Both chromosomal and plasmid-borne exon 4s of mod(mdg4) are trans-spliced to the chromosomal 3′ exons of lola (left) and vice versa (right). Trans-spliced products formed between chromosomal exons (endo–endo) and between plasmid-borne and chromosomal exons (exo–endo) are indicated. Boxes in colors are used similarly to in other figures.