Figure 2.

Expression of other RPE cell markers. (A) RPE cells were examined for expression of VCAM‐1, VEGF‐R1, VEGF‐R2, Endoglin, ICAM‐1, ICAM‐2, CD36, PDGF‐Rα, PDGF‐Rβ, and CD47by FACS analysis. The shaded area shows control IgG staining. (B) Cell lysates were prepared from wild‐type (WT), PEDF−/−, and TSP1−/− RPE cells and analyzed for expression level of MFG‐E8, PDI, ICAM‐1 and β‐actin as loading control by Western blot analysis using specific antibodies. The quantitative assessment of data is shown on the right (*P < 0.05, ***P < 0.001, n = 3).