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. 2015 Jan 19;3(1):e12266. doi: 10.14814/phy2.12266

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© 2015 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — Attenuation of migration in PEDF−/− and TSP1−/− RPE cells. (A) Scratch wound assay of RPE cell monolayers on gelatin‐coated plates was used to determine cell migration. Wound closure was observed by phase microscopy at indicated time points. (B) The quantitative assessment of wound migration (**P < 0.01, ***P < 0.001, n = 3). (C) Migration of RPE cells in transwell assays. Please note a significant decrease in the migration of PEDF−/− and TSP1−/− RPE cells compared with WT cells (***P < 0.001, n = 3). (D and E) The re‐expression of PEDF and TSP1 in PEDF−/− and TSP1−/− RPE cells. PEDF−/− and TSP1−/− cells were infected with viruses encoding PEDF or TSP1 cDNAs as detailed in Material and Methods. The expression of PEDF and TSP1 was confirmed by Western blot analysis. These lanes are all from the same membrane and extraneous lanes were removed for uniform labeling of the Figure. (F) Reexpression of PEDF and TSP1 in PEDF−/− and TSP1−/− RPE cells restored their migration to near WT RPE cell as determined by transwell migration assay (*P < 0.05, ****P < 0.0001, n = 3).