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. 2015 Jan 19;3(1):e12246. doi: 10.14814/phy2.12246

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© 2015 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — Illustration of workflow used for constant‐current electroporation in vitro. (A) Recording pipette is moved into gentle contact with the target cell under optical guidance. (B) Immediately prior to electroporation Rs is measured using the amplifier bridge‐balance function and (C) used to calculate the electroporation current (I_e) required to generate the target voltage (V_t; in this example 7.5 V). (D) The amplifier is programmed to deliver a train of current pulses at I_e.