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. 2015 Jan 27;3(1):e12272. doi: 10.14814/phy2.12272

Figure 2.

Figure 2.

Expression of endothelial nitric oxide synthase (eNOS) in the HSPG2−/−‐Tg aorta. RNA expression levels of von Willebrand factor and eNOS in the aortic tissues of 10 week control and HSPG2−/−‐Tg mice were analyzed using qPCR. (A) The expression of von Willebrand factor was not significantly different, while (B) eNOS expression was significantly reduced in the HSPG2−/−‐Tg animals (n = 6 per genotype. The bars indicate the mean ± SEM). RNA expressions levels were normalized to that of β‐actin and were indicated as relative to β‐actin. (C) The protein expression levels of eNOS and β‐actin in the aortic tissues were evaluated, using Western blotting. Each band was quantified using ImageJ software and is shown as relative to β‐actin. The protein expression levels of eNOS were significantly decreased in the HSPG2−/−‐Tg mice compared to that in the control mice (n = 4 per genotype. The bars indicate the mean ± SEM). *P < 0.05 versus control mice.