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. 2015 Jan 27;3(1):e12272. doi: 10.14814/phy2.12272

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© 2015 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — RNA expression level of eNOS in Perlecan knockdown HAECs on perlecan recombinant protein. qPCR analysis of eNOS RNA expression level in HAECs treated with Perlecan or Control siRNA and seeded on a plate coated with or without perlecan recombinant protein (rPerlecan). The level of the eNOS RNA expression was normalized to that of GAPDH and it is indicated as relative to GAPDH. The eNOS expression level in HAECs treated with Perlecan siRNA was significantly decreased compared to that of Control siRNA treated HAECs. HAECs treated with Perlecan siRNA and plated on rPerlecan was no significantly different compared with that of Control siRNA treated HAECs. The bars indicate the mean ± SEM (n = 4) ***P < 0.001 versus Control siRNA treated HAECs without rPerlecan, ^###P < 0.001 versus Perlecan siRNA treated HAECs without rPerlecan.