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Stx1, Stx2, and histones induce changes in renal glomerular endothelial expression of surface molecules. Surface expression of (A) PAR1, (B) EPCR, and (C) TM were quantified by on-cell western after 24 h incubation with Stx1 (50 ng/mL), Stx2 (100 ng/L), or 50 μg/mL histones. Antigen data were normalized to cell number using Draq5 staining. Differences from media alone are shown as ***p < 0.001.
