Table 2. Human CECs expansion profile with and without the presence of Y-27632.

Donor	OD Control @ P2	OS ROCKi @ P2	Fold Increase
17	260,000	510,000	1.96
22	1,845,000	4,680,000	2.54
23	617,000	2,070,000	3.36
24	708,000	1,890,000	2.67

Expansion profiles of primary human CECs propagated to the second passage were compared. Estimated cell-yield of CECs expanded using the dual media approach as control (OD) was compared to donor-matched CECs grown in the dual media approach supplemented with Y-27632 throughout the study (OS). An increase of at least 1.96 fold and up to 3.36 fold was observed in studies conducted using CECs isolated from 4 independent donors.