Table 5. Information of primary antibody used in this study.

Antibody (Clone)	Company (Catalog Number)	Fixative	Concentration
Na+/K+-ATPase (0.T.1)	Santa Cruz (sc-71638)	100% Ethanol 5 minutes, 4°C	5 μg/mL
ZO-1 (1/ZO-1)	BD Pharmingen (610966)	100% Ethanol 5 minutes, 4°C	5 μg/mL
Glypican-4 (MAFB4044)	Creative Biomart (CAB-244MH)	Fix and Perm (Medium A) 15 minutes, room temp.	10 μg/mL
CD-200 (MRC OX-104)	BD Pharmingen (552023)	4% freshly made PFA 10 minutes, 4°C	10 μg/mL

The various primary antibodies used in this study are listed here by their clone name, the company of which the primary antibody was purchased from, with their respective catalog numbers. The different methods of fixation and concentration used were optimized for the staining of primary human CECs.