Figure 5.

Effects of IL-17A and/or TNF-α on BV/TV ratio. Bone samples were cultured for 7 days in six well-plates, in the presence or absence of IL-17A (50 ng/ml) and TNF-α (1 ng/ml) or both, then bone samples were used for histomorphometric analysis. Bone volume over tissue volume (BV/TV) was measured with an automatic image analyzer at low power field. (A) Results were analyzed using the Wilcoxon test. *p < 0.05 vs. without cytokines. The bars of each graph represent mean + SEM. (B) Representative histomorphometric sections from OA and RA bone samples with and without the combination of IL-17A and TNF-α. Goldner’s staining shows trabecular bone in green (10× magnification).