(a) PCR primers and conditions

Region	Position*	Sequence#	Conditions of both reactions	PCR product
Promoter	4421-4446 4808-4830	5′-GAAAATGCTTACCCAG(G)ACAAGCCTGT-3′  5′-GTCTCCTCATATCCCCAGGC-3′	94°C for 5 min, followed by 30 cycles (94°C for 30 s, 64°C for 30 s, and 72°C for 30 s) and a final elongation at 72°C for 5 min	134 bp
Exon 1	5191-5218 5305-5324	5′-CATCAACGGCTTCCCAGG(C)G(A)CAAGATGGG-3′  5′-GTCTCCTCATATCCCCAGGC-3′		410 bp