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. 2015 Apr 7;9:109. doi: 10.3389/fncel.2015.00109

Figure 1.

Figure 1

Amyloid precursor protein increases in LAMP1- and Rab7-positive vesicles. (A–G) Confocal microscopy analysis of double-staining of mouse anti-APP [red; (A,E)] and rabbit anti-Lamp1 [green; (B,F)] in WT and APPYG/YG (YG) hippocampal neurons [(A–D) and (E–H), respectively; 63× objective). Scale bars = 10 μm. The panels are representative of five different experiments performed in duplicate. [(C,G) and (D,H)] Co-localization analysis of WT and APPYG/YG hippocampal neurons. The analysis was performed using Zen/Zeiss software. Notice that the area of overlap between APP and Lamp1 immunostaining (white) was significantly increased in APPYG/YG mice (H) compared with WT (D). (D,H) High magnification of (C,G). The (R) coefficient (Pearson’s coefficient) was used for the quantitative and comparative analyses (I). The data are expressed as mean ± SEM. n = 10. *p < 0.05. (J–P) Confocal microscopy analysis of double-staining for mouse anti-APP [red; (J,N)] and rabbit anti-Rab7 [green; (K,O)] in WT and APPYG/YG (YG) hippocampal neurons [(J–M) and (N–Q), respectively; 63× objective]. Scale bars = 10 μm. The panels are representative of four different experiments performed in duplicate. [(L,P) and (M,Q)] Co-localization analysis of WT and APPYG/YG hippocampal neurons. The analysis was performed using Zen software. Notice that the area of overlap between APP and Rab7 immunostaining (white) was significantly increased in APPYG/YG mice (Q) compared with WT (M). (M,Q) High magnification of (L,P). The (R) coefficient (Pearson’s coefficient) was used for the quantitative and comparative analyses (R). The data are expressed as mean ± SEM. n = 8. *p < 0.05.