Table 2.
Stock, IS, standard, sample, and blank solutions preparation.
| Composition and process | Stock solution of standards | Stock solution of IS | Standard solution | Sample solution | Blank solution |
|---|---|---|---|---|---|
| Sotalol hydrochloride | ~100.00 mg | — | — | — | — |
| Potassium sorbate | ~20.00 mg | — | — | — | — |
| Ethylparaben | — | ~100.00 mg | — | — | — |
| Stock solution of standards | — | — | 1.000 mL | — | — |
| Stock solution of IS | — | — | 1.000 mL | 1.000 mL | — |
| Oral preparation (SOT 5 mg mL−1) | — | — | — | 1.000 mL | — |
| Placebo of preparation (neither SOT nor SORB) | — | — | — | — | 1.000 mL |
| Dissolvent ACN : water | 50 : 50 (v/v) | 50 : 50 (v/v) | 30 : 70 (v/v) | 30 : 70 (v/v) | 30 : 70 (v/v) |
| Total volume | 20.00 mL | 50.00 mL | 25.00 mL | 25.00 mL | 25.00 mL |
| Membrane filtration 0.22 µm | — | — | Yes | Yes | Yes |
| Injection to the column | — | — | Yes (5 µL) | Yes (5 µL) | Yes (5 µL) |