Figure 2.
Effects of dispersed single-walled carbon nanotubes on cytotoxicity and level of cellular uptake in macrophages.
Notes: (A) Cells (2×104 cells/well in 96-well plates) were treated with various concentrations of dispersed swCNTs. After 24 hours of treatment, cell viability was determined using the MTT assay. Hydrogen peroxide (500 μM) was used as a positive control. Cell viability was determined by the relative absorbance compared to control. (B, C) Cells (2×105 cells/well in 4-well plates) were treated with 1 μg/mL swCNTs for 24 hours. After treatment with swCNTs, the cells were stained with F-actin (green) and DAPI (blue). Images showed intracellular location of swCNTs (black). The fluorescence was visualized using (B) densitometric analysis and (C) confocal microscopy (×400). The results are presented as mean ± SE of three independent experiments. *P<0.05 significantly different from control.
Abbreviations: CON, control; DAPI, 4′,6-diamidino-2-phenylindole; MTT, 3(4, 5-dimethylthiazolyl-2)2,5-diphenyl tetrazolium bromide; PBS, phosphate-buffered saline; SE, standard error; swCNT, single-walled carbon nanotube; XPS, X-ray photoelectron spectroscopy.