Figure 3.

Effects of dispersed single-walled carbon nanotubes on oxidative stress.

Notes: (A) Cells (2×10⁴ cells/well in 96-well plates) were treated with 1 μg/mL swCNTs for 24 hours. After treatment, the cells were stained with 10 μM DHR 123 for 30 minutes. Production of ROS was determined using DHR 123 staining. The fluorescent intensity of DHR was recorded using a fluorescent plate reader. (B) Cells (2×10⁶ cells/well in 6-well plates) were treated with 1 μg/mL swCNTs for 24 hours. Superoxide dismutase (SOD) activity was determined using a tetrazolium-based SOD assay kit. FeSO₄ (100 μM) was used as a positive control. The results are presented as mean ± SE of three independent experiments. *P<0.05 significantly different from control.

Abbreviations: CON, control; DHR, dihydrorhodamine; ROS, reactive oxygen species; SE, standard error; SOD, superoxide dismutase; swCNT, single-walled carbon nanotube.