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| Method | Vapour-diffusion (hanging-drop) |
| Plate type | 24-well Linbro Plate |
| Temperature (C) | 20 |
| Protein concentration (mgml1) | 7 |
| Buffer composition of protein solution | 35mM K2HPO4/KH2PO4 pH 7.0, 175mM NaCl |
| Composition of drop solution | 20%(w/v) PEG 3350, 0.2M ammonium nitrate |
| Composition of reservoir solution | 26%(w/v) PEG 3350 |
| Volume and ratio of drop | 2l (1l protein solution + 1l precipitant) |
| Volume of reservoir (ml) | 1 |
