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. Author manuscript; available in PMC: 2015 Apr 7.
Published in final edited form as: Biochim Biophys Acta. 2014 Nov 18;1853(2):276–284. doi: 10.1016/j.bbamcr.2014.11.015

Fig. 5.

Fig. 5

Increased sensitivity of H9c2 cells to hypoxia compared to HL-1 cells. A–D: Representative phase contrast microscopy of H9c2 and HL-1 cells. Representative phase contrast pictures were taken before (A, C) and after (B, D) hypoxic treatment (0. 4% oxygen) for 16 h and reoxygenation for 24 h. H9c2 cells were almost completely lysed after hypoxia, whereas HL-1 substantially survived after the same hypoxic regime. HL-1 and H9c2 cells were seeded in 6-well plates and grown overnight under their corresponding conditions. E: Quantitative data of cell viability after 16 h and 24 h of hypoxia. N = 3; * P < 0.05; *** P < 0.001.