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. Author manuscript; available in PMC: 2015 Apr 7.
Published in final edited form as: Mol Carcinog. 2013 Apr 26;53(10):787–792. doi: 10.1002/mc.22034

Figure 1.

Figure 1

Cten protein levels are regulated by EGF, FGF2, NGF, TGF-β, PDGF, IGF-1, IL-6, and IL-13 in human prostatic epithelial cells. MLC-SV40 cells cultured in plain medium (without EGF and bovine pituitary extract) for 24 h were treated with indicated concentrations of growth factors or cytokines for another 24 h (A) or treated with indicated concentration for 1, 3, and 6 h (B). Total cell lysates were prepared and protein expression levels were monitored by immunoblotting with indicated antibodies. GAPDH was detected as an equal loading control. The intensity of each cten band was quantified, and expressed as a fold increase using the signal at dose 0 or time 0 as a reference.