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. Author manuscript; available in PMC: 2015 Apr 7.
Published in final edited form as: Mol Carcinog. 2013 Apr 26;53(10):787–792. doi: 10.1002/mc.22034

Figure 4.

Figure 4

Mek-Erk and PI3K-Akt are two main pathways involved in growth factor-mediated cten expression. (A) MLC-SV40 or SW480 cells cultured in plain medium for 24 h were pre-treated with indicated inhibitors for 30 min prior to FGF2, NGF, IL-13, IL-6, IGF-1, PDGF, or TGF-β 24 h incubation. Cten protein levels were determined by immunoblotting. C, control (no inhibitor); AG, AG490 (JAK2 inhibitor, 50 μM); U, U0126 (Mek1/2 inhibitor, 10 μM); LY, LY294002 (PI3K inhibitor, 25 μM); PD, PD98059 (Mek1 inhibitor, 25 μM). (B) Cell lysates prepared from SW480 transfected with control (con), constitutive active Mek1 (CA-Mek1), Mek2 (CA-Mek2), myr-PI3KCA (PI3KCA), Akt-T308D/S473D (Akt) were immunoblotted with indicated antibodies.