FIGURE 7.
FAK−/− neutrophils are defective in killing of E. coli in vitro and in vivo. A, Wild-type (WT) and FAK−/− (KO) neutrophils were incubated with serum-opsonized E. coli at 37°C for 30 and 120 min, and the numbers of surviving bacteria were determined and presented as CFUs. B and C, Wild-type and FAK−/− mice were injected i.p. with 2 × 106 E. coli in 0.9% NaCl. After 4 h, mice were killed, and the peritoneal lavage fluid was collected. Surviving bacteria were enumerated by plating several dilutions (1/500, 1/1000, and 1/5000) onto LB agar (B) and total numbers of surviving bacteria per animal were calculated (C). Results are means ± SD of four mice. *, p < 0.05; **, p < 0.01 vs wild-type neutrophils by Student’s t test. ■, Wild type; □, FAK−/−.