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. Author manuscript; available in PMC: 2015 Apr 7.
Published in final edited form as: J Immunol. 2009 Jun 26;183(2):1032–1043. doi: 10.4049/jimmunol.0802984

FIGURE 8.

FIGURE 8

FAK deletion enhances spontaneous neutrophil death. A, Bone marrow-derived wild-type (WT) and FAK−/− (KO) neutrophils were cultured in RPMI 1640 containing 10% heat-inactivated FBS at a density of 5 × 105 cells/ml. Apoptotic cells were detected by annexin V-FITC staining and 7-aminoactinomycin D (7-AAD) staining. Ten thousand cells were collected at the indicated time points and analyzed by FlowJo software. Region R1, viable cell; Region R2, early apoptotic cells; Regions R3 and R4, late apoptotic cells and necrotic cells. B, Time course of neutrophil spontaneous death. Results are means ± SD of two separate experiments.