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. 2015 Mar 27;71(Pt 4):969–985. doi: 10.1107/S1399004715002485

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© Alonso-García et al. 2015

This is an open-access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.

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Paramagnetic labels attached to the FnIII-3 and FnIII-4 domains of β4. (a) The predicted average locations of the nitroxide group of MTSL attached to Cys are shown as spheres in the crystal structure of FnIII-3. The positions that yielded well defined inter-spin distances with a second probe at C1608 are coloured green. Those that resulted in broad distance distributions but did not alter the global structure of FnIII-3,4 are coloured magenta. The positions that resulted in a distortion of the structure are coloured dark violet. (b) Crystal structure of FnIII-4 with the estimated average position of the paramagnetic groups. The positions that yielded useful distances are coloured green, while those that resulted in broad distributions are shown in pink.