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DEER results for mutants containing C1559 in the interdomain linker. (a) Normalized dipolar evolution (black lines) and fits to the data (grey lines) for two doubly labelled mutants that include C1559. (b) Distance distribution profiles calculated from the data in (a) (solid line) and a calculated distribution for one of the final models (dashed line). See Supplementary Fig. S10 for analysis of these proteins by SAXS.
