Table 2. SAXS data collection and derived parameters for 4 FnIII-3,4.
| Data-collection parameters | |
| Instrument | EMBL P12 beamline, PETRA, DESY, Hamburg |
| Beam geometry | 0.2 0.12mm |
| Wavelength () | 1.24 |
| q range (1) | 0.010.35 |
| Exposure time (s) | 20 0.05 |
| Concentration range (mgml1) | 1.548.6 |
| Temperature (K) | 283 |
| Structural parameters† | |
| I(0)/c (102cm2mg1) (from Guinier) | 1.24 0.01 |
| R g () (from Guinier) | 22.1 0.8 |
| I(0) (102cm2mg1) [from P(r)] | 1.23 0.01 |
| R g () [from P(r)] | 22.0 |
| D max () | 70 |
| Porod volume estimate V p (3) | 32000 1000 |
| Excluded volume estimate V ex (3) | 42000 200 |
| Molecular-mass determination | |
| I(0) (102cm2mg1) | 4.20 0.01 |
| Molecular mass M r (Da) [from I(0)] | 19200 |
| Molecular mass M r (Da) [from (V p/1.6)] | 20000 625 |
| Molecular mass M r (Da) [from (V ex/2)] | 21000 100 |
| Calculated monomeric M r from sequence | 23360 |
| Software used | |
| Primary data reduction | Automated SAXS pipeline |
| Data processing | PRIMUS (Konarev et al., 2003 ▶) |
| Ab initio analysis | DAMMIF (Franke Svergun, 2009 ▶), DALAI_GA (Chacn et al., 2000 ▶) |
| Validation and averaging | SUPCOMB (Kozin Svergun, 2001 ▶) DAMAVER (Volkov Svergun, 2003 ▶) |
| Computation of model intensities | CRYSOL (Svergun et al., 1995 ▶) |
| Three-dimensional graphical representations | PyMOL (Schrdinger) |
| SASBDB code | SASDAT6 |
†
Values reported for the data extrapolated to infinite dilution.
‡
Absolute intensities determined using water as a secondary standard (Orthaber et al., 2000 ▶).
§
Calculated using BSA as a standard (66000Da).