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TesB activity against a range of substrates (a), demonstrating activity against a broad range of fatty acyl-CoA chain lengths; octanoyl-CoA was identified as the preferred substrate and was investigated further. (b) The active site with CoA and LDAO (from the EcTesB model) superimposed. (c) Activity curve for octanoyl-CoA, with a Hill coefficient of 1.753 and a V max of 478 µmol min−1 mg−1.
