Skip to main content
. Author manuscript; available in PMC: 2015 Apr 7.
Published in final edited form as: Genes Cells. 2013 Nov 19;19(1):28–51. doi: 10.1111/gtc.12104

Figure 6. The AD1 domain of CoCoA interacts with CCAR1(501–691), while the AD2 domain of CoCoA interacts with the GATA1 CF domain.

Figure 6

Figure 6

(A, B, E, G) Mammalian two-hybrid assays. The luciferase activity of the reporter with 5×Gal4-binding sites was measured. The interaction between various Gal4-fusion fragments of CoCoA (A, B, E, G) and various VP16-fusion fragments of CCAR1 (A, B) or GATA1 (E, G) were tested. Values (mean ± SD) of a representative experiment performed in duplicate are shown. The results were reproducible in three independent experiments.

(C, D, F) GST-pull down assays. HA-CoCoA (C, D) or FLAG-GATA1 (F) was pulled down by various GST-fusion fragments of GATA1 (C, D) or CoCoA (F). HA-CoCoA and FLAG-GATA1 were visualized using an anti-HA or -FLAG antibody, respectively.

(H) Diagram of CoCoA showing the activation domains AD1 and AD2, the coiled-coil domain, and the leucine zippers. Transcription factors that interact with these domains are also shown.