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HPLC analysis of the conversion of UDP-d-glucose and sulfite to UDP-sulfoquinovose by Agl3 (a) UDP-sulfoquinovose biosynthesis was analyzed under sulfite-saturated (graph 1) and unsaturated conditions (graph 2). b The UDP-d-glucose-5,6-ene is present at sulfite-saturated UDP-sulfoquinovose biosynthesis (graph 1; only trace amounts), and under unsaturated conditions (graph 2). c dehydration of UDP-d-glucose is a key catalytic activity of Agl3
