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Conversion of UDP-d-glucose to UDP-d-glucose-5,6-ene by Agl3 at an excess of deuterium. a Conversion of UDP-d-glucose to UDP-d-glucose-5,6-ene reaching an equilibrium reaction at steady-state level. b MS analysis of the conversion of UDP-d-glucose to UDP-d-glucose-5,6-ene in H2O-phosphate buffer compared to (c) its conversion in D2O-phosphate buffer. d Natural isotope distribution pattern of UDP-d-glucose measured by ESI–MS in the negative mode in H2O-phosphate
