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Proposed mechanism for the conversion of UDP-d-glucose to UDP-d-glucose-5,6-ene (a) NAD-dependent oxidation of UDP-d-glucose at C-4 (structure 3), enolisation of UDP-4-keto-glucose (structure 4) and locking of deuterium at C-5 by the enolisation process at the active site of Agl3 (structures 5–7). b Proposed NAD-dependent dehydration of the enol-conformation of UDP-4-keto-glucose eventually resulting in UDP-d-glucose-5,6-ene. 160 × 135 mm (300 × 300 DPI)
