Table 1.

Summary of Crn1-DCC binding affinities for WT and mutant yeast actins

	Kd (µM)	Kd/KdWT	Coronin residues in close proximity
WT	0.87 ± 0.04	1.00
DNEQ*	0.81 ± 0.01	~ 1
ΔDSE**	0.76 ± 0.03	~1
K50A/D51A (red***)	1.55 ± 0.06	~2	D250 and D273
D80A/D81A (magenta)	1.9 ± 0.5	~2	R323, K295
E83A/K84A (blue)	5.8 ± 0.3	~7	K227, D297
E99A/E100A (cyan)	16	~18	K204, K247
K315A/E316A (green)	1.4 ± 0.1	~1.6	K20, K21 and E22 (with actin “i+1”)
D363A/E364A (yellow)	4.4 ± 0.8	~5	R141, R142, and R202 (with actin “i”) R361 and R362 (with actin “i+2”)
R37A/R39A (orange) +Phalloidin ****	2.7 ± 0.1	~3	E320
WT+150 mM KCl	2.3	~2–3

^*- D2N/E4Q actin mutant with a neutralized N-terminus.

^**ΔDSE – mutant actin with its three N-terminal residues deleted

^***Color names in parentheses refer to the colors of these residues in Supplementary Fig. 5

^****This set of measurement was carried out in the presence of equimolar concentrations of phalloidin.