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. Author manuscript; available in PMC: 2015 Apr 7.
Published in final edited form as: J Immunol. 2011 Jan 14;186(4):2117–2126. doi: 10.4049/jimmunol.1002337

FIGURE 2.

FIGURE 2

Anti-TNF-α antibody decreases inflammatory mediators during elicitation of CHS. Groups of 4 C57BL/6 mice were sensitized with 0.25% DNFB on days 0 and +1. On day +5, the indicated groups of sensitized and non-sensitized naïve mice were treated with 250 μg anti-TNF-α mAb prior to challenge with 0.2% DNFB. A–C. Challenged skin was excised at 1, 2, 3, and 4 hrs. post-challenge and snap-frozen. Skin from naïve/non-challenged (NNC) mice was excised as a control. Whole cell RNA was prepared and was used to assess mRNA expression of ICAM-1, IL-17, and IFN-γ in the skin samples by quantitative RT/PCR. The mean expression level for each of 4 samples per group ± SEM is shown. D. Ear thickness was monitored pre-challenge and 24 hr. after challenge. The mean increase in ear thickness following hapten challenge is shown in 10-4 in. ± SEM for groups of 4 mice. All results are representative of two individual experiments each. All results are representative of two individual experiments. *p ≤ 0.05.