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. Author manuscript; available in PMC: 2015 Apr 7.
Published in final edited form as: J Immunol. 2011 Jan 14;186(4):2117–2126. doi: 10.4049/jimmunol.1002337

FIGURE 3.

FIGURE 3

Neutralization of ICAM-1 or LFA-1 during skin challenge to elicit CHS inhibits CXCL1 production and neutrophil infiltration into the challenge site. Groups of 4 C57B/6 mice were sensitized with 0.25% DNFB on days 0 and +1. On day +5, sensitized mice were treated with either 300 μg anti-ICAM-1 mAb, 300 μg anti-LFA-1 mAb, a mixture of 150 μg of both antibodies, or 300 μg control rat IgG and immediately challenged with 0.2% DNFB. A. Challenged areas of skin were removed at 6 h post-challenge from sensitized treated mice and non-sensitized mice and prepared tissue homogenates were tested for the levels of CXCL1 by ELISA. The mean concentration ± SEM for 4 individual samples per group is shown. All results are representative of two individual experiments each. *p ≤ 0.04. B. The skin was excised 6 h after challenge, digested, and prepared cell suspensions were stained with FITC-labeled anti-CD45 mAb and PE-labeled anti-GR-1 mAb and analyzed by flow cytometry to assess neutrophil infiltration into the challenge site. All results are representative of two individual experiments each.