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. 2015 Apr 7;10(4):e0122933. doi: 10.1371/journal.pone.0122933

Fig 2. Tobacco seedling responses to a high temperature challenge.

Fig 2

(A) Photograph of segregating SR1-GS24 F2 tobacco seedlings that had been heat treated for 5 min at 50°C and returned to the dark at 22°C for 16 hours. The seedlings with the asterisk above them were negative when analyzed for AtHSP101 by PCR. (B) PCR analysis of the segregating SR1-GS24 F2 tobacco seedlings shown in (A) using P-AGS-> HS101-5’<- primers. (C) Photograph of the characterization of tobacco heat tolerance using the hypocotyl elongation assay on SR1 (AtHSP101 minus) and SR1-GS2 (AtHSP101 plus) seedlings following a 5 min 50°C heat challenge and 16 h recovery. These assays were used to check for line homogeneity.