Fig 4. Photographs of RT-PCR and Western Blot analysis showing the expression of AtHSP101 and presence of HSP101 proteins in transgenic cotton lines.
RT-PCR products from pollen (A) and leaf (B) tissues of transgenic line 40 plants grown in 31°C/27°C greenhouse; Western blot analysis of HSP101 for: mature pollen of transgenic line 40 (C) and null line 52 (F, AtHSP101 minus) plants grown in a greenhouse set to 31°C/27°C day/night; leaf tissues of transgenic line 40 (D) and null line 52 (G) plants grown in a greenhouse set to 31C°/27°C day/night; leaf tissues of transgenic line 40 (E) and null line 52 (H) plants grown in a hot greenhouse set to 43°C/28°C day/night temperatures.