Table 1. Characterization of the heat tolerance of AtHSP101 plus and AtHSP101 minus cotton plants from different cell lines.

Treatment	Cell Line	Transgenic Plant Number	Bolls/Plant	Seeds/Plant	Seeds/Boll
43°C/28°C day/night	1–4 (pos.)	1	50	482	9.64
	1–4 (pos.)	2	51	418	8.20
	1–8 (pos.)	3	27	286	10.59
	1–8 (pos.)	22	29	251	8.66
	1–8 (pos.)	24	43	283	6.58
	21–2 (pos.)	5	18	142	7.89
	Average		36	310	8.59
	21–2 (neg.)	4	17	60	3.53
	C312-5A (neg.)	8	13	191	14.69
	6–9 (neg.)	10	10	110	11.00
	C312-5A (neg.)	18	23	220	9.57
	Average		12	240	9.70
31°C /27°C day/night	1–4 (pos.)	51	12	240	20
	1–4 (pos.)	50	18	424	23.56
	1–4 (pos.)	48	15	362	24.13
	1–8 (pos.)	40	22	631	28.68
	21–2 (pos.)	34	18	513	28.5
	21–2 (pos.)	35	31	703	22.68
	Average		19	479	25.2
	1–8 (neg.)	39	24	710	29.58
	1–8 (neg.)	52	14	352	25.14
	2–6 (neg.)	31	32	756	23.63
	Average		23	606	26.35

Values in boldface within a treatment are significant at a = 0.05 comparing AtHSP101 positive and AtHSP101 negative plants. Values underlined are significant at a = 0.05 comparing AtHSP101 positive and AtHSP101 negative plants between treatments.

Plants were grown in either a greenhouse with control (31°C /27°C day/night) or heat-treated (43°C/28°C day/night) environments.