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. 2015 Apr 7;10(4):e0123138. doi: 10.1371/journal.pone.0123138

Fig 2. Mass spectrometry confirms the presence of acrolein adducts on HDL apolipoproteins.

Fig 2

HDL was incubated with 250 μM acrolein for 24 hours. Intact protein was analyzed with a Waters Q-TOF ApCI US quadrupole/time-of-flight mass spectrometer interfaced to a CapLC liquid chromatograph through a Advion Nanomate electrospray source. Mass spectrometry analysis of (A, B) apoA-I on native HDL; (C, D) apoA-I on acro-HDL; and (E, F) apoA-II on acro-HDL are shown. Brackets indicate molecular weight shifts corresponding to acrolein adduct formation. An extended view of masses up to 100,000 Da are shown (Panels B, D, F).