Fig 5. HDL modification by acrolein leads to decreased selective uptake efficiency of HDL-COE.

SR-BI-expressing COS-7 cells were incubated with double-radiolabeled HDL or acro-HDL and selective uptake efficiency for each ligand was calculated as a function of ligand concentration (panel A; for 1.5 h at 37°C) or incubation time (panel B; ligand concentration of 10 μg/mL at 37°C). Values for binding to SR-BI and selective uptake of COE are shown in Table 1. Values represent the mean ± SEM of at least four independent experiments, each performed in triplicate. Data sets are presented following subtraction of empty vector values. Statistical analyses were determined by paired t-test. *p<0.05, **p<0.01 and ***p<0.001